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you will find tyrosine (Y223) and lysine (K139) residues that might also be contributing towards the interaction With all the phosphate moiety of the substrate. Deprotonation of either of such residues at large pH could bring about a fall in the value of k
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This observation indicates that there has been a divergence in the evolution of NagA and indicates that there are essential variances while in the mechanistic details for substrate activation and hydrolysis. NagA from E. coli
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m vs. pH-profile for Zn-NagA is shown in Determine 2B. With this profile, catalytic action is dropped at higher and small values of pH and the info were being suit to naga26 equation 4 where two teams, in both the substrate or free enzyme, with identical p